Rome, May 20 - New video showing Mafia kingpin Bernardo Provenzano looking old and frail behind prison bars has triggered new complaints about his case. The video clips, aired on a public affairs program on television network LA7, are raising doubts about the mental and physical health of Provenzano. Taken from surveillance cameras inside a top-security prison in the Italian city of Parma, the TV program reiterates his family's concerns about ill treatment of the Cosa Nostra boss, convicted on numerous counts of murder. A request for his release on health reasons was refused last month. Arrested in 2006 after 43 years on the run, Provenzano has been subjected to the so-called 41-bis treatment for Italy's most dangerous criminals. They can be kept in single-person cells in maximum-security jails, almost entirely cut off from the outside world in order to prevent further criminal activity. The ex-Mafia chieftain, once nicknamed "The Bulldozer", is serving life for various murders including ordering fatal bomb attacks on anti-mafia prosecutors Giovanni Falcone and Paolo Borsellino in 1992.