(ANSA) - Milan, October 15 - Rome's prosecutor is appealing the acquittal last summer of former Premier Silvio Berlusconi, his son Pier Silvio Berlusconi, and other defendants in a case of alleged fraud at a subsidiary of his Mediaset broadcasting empire. The appeal to Italy's Supreme Court was filed Monday by prosecutor Fabio De Pasquale in Milan. It comes after a preliminary hearings judge in Rome last June declared that the case against Berlusconi and 10 others had been timed out under the statute of limitations. Berlusconi has already been acquitted of a similar case in Milan, also concerning alleged wrongdoing in the trading of film rights by the Mediatrade subsidiary. The other defendants in this case include Egyptian-born director and producer Frank Agrama. In this and several other previous trials, Berlusconi has always denied wrongdoing, claiming he is the victim of a minority group of allegedly left-wing prosecutors and judges who he says are persecuting him for political reasons. In more than a dozen cases, the ex-premier has never received a definitive conviction, sometimes because of law changes passed by his governments, while some other charges were timed out.